How do CO2 incubators work
Experiments that imitate processes in living organisms are called in vitro. In these, cells and tissue cultures are grown in the laboratory, often over several weeks, in an environment that is as natural as possible.
Cultivation takes place in a CO2 incubator, in which not only the temperature, but also the humidity and carbon dioxide content, must be controllable. In addition to this, the oxygen and nitrogen content can also be adjusted in some appliances.
The CO2 incubator usually has a chamber volume of between 50 and 300 liters and a temperature range from +4 ℃ and +8 ° C above room temperature to +60 ° C (details on the basic operation and features of a temperature control chamber). The interiors of CO2 incubators are normally made of corrosion-free stainless steel 1.4301 (acc. To standard ASTM 304) and they are additionally smoothed with electrolytic polishing by some manufacturers to prevent germs from settling and to make cleaning easier. In order to observe the chamber load without affecting the atmosphere inside the chamber, CO2 incubators are usually equipped with an inner glass door or additional gas baffle.
Regulating air humidity
Normal temperatures in a CO2 incubator lie at around the same temperature as the human body (37 ° C). The essential matter is maintaining humidity in a controlled manner, while at the same time avoiding condensation in the interior. This is done in practice either through humidification via water trays in the interior of the CO2 incubator, via direct contact of water with the floor or through an activity controllable system for humidification and dehumidification in which the water supply lies outside the working chamber. The water from the external water tank is heated up in a vaporator and fed into the chamber as steam, which means that only sterile water (pyrolytic germ barrier) humidifies the chamber and the samples and the humidity can be applied in specific doses.
The active control of humidity guarantees that the required humidity is reached quickly after the door is opened and closed (see Chapter DIN 12880: 2007-05).
Heating the chamber
Precisely maintaining the atmosphere is absolutely essential in a CO2 incubator to protect samples. This is why there is a whole range of different heating systems in practice that are supported in part by motorized fans, and in part by air circulation systems free of turbulence.
Many CO2 incubators are supported by a heated air jacket, and in some cases by a water jacket, ensuring even temperature distribution and temperature stability, rapid recovery times after the door has been opened and maintenance of the temperature after a power failure. At the same time, the air jacket prevents condensation from forming. Some manufacturers, who do without a jacket of air or water, instead heat the chamber directly from all six sides, surrounding it in addition with an insulation coat. In this form of direct heating, a high degree of flexibility and a high density in the arrangement of heating elements form the basis of temperature homogeneity.
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